Q: Does ASMCF provide services for sample preparation and data analysis?
A: No, ASMCF primarily provides instrument services for MS analysis of small molecules. If you have needs for sample preparation or data analysis, we recommend collaborating with researchers experienced in these areas (Advanced request; https://msomics.abrc.sinica.edu.tw/ASMCF/index.php/workflow/). Alternatively, you can seek collaboration through the Expert Committee of the ASMCF (https://msomics.abrc.sinica.edu.tw/ASMCF/index.php/expert-committee/).
Q: How does ASMCF charge for its services? Can you provide a quote before service?
A: ASMCF charges based on the actual usage time of the instrument analysis. Since the usage time may vary depending on the condition of sample loading, it’s not possible to provide an accurate quote before the analysis.
Q: What sample preparation methods does ASMCF recommend?
A: Due to significant differences in the physical and chemical properties of metabolites, there isn’t a single extraction method or analysis platform that can cover all metabolites. The choice of extraction method and analysis platform is typically based on the sample’s characteristics and whether there are specific categories of metabolites you want to prioritize for detection. For metabolomics profiling, we typically employ a general protocol for metabolites extraction, primarily using the combination of methanol, chloroform, and water (https://msomics.abrc.sinica.edu.tw/ASMCF/index.php/extraction-protocol/).
In the case of targeted analysis, it is advisable to explore specific protocols that offer better extraction efficiency for targeted metabolites.
Q: What analysis platform does ASMCF recommend?
A: For general metabolites, we have three commonly used analytical columns: HILIC for polar metabolites, T3 for polar-middle polar metabolites, and C18 for non-polar metabolites (mainly lipids). Besides these, if there are other analytical columns you’d like to discuss, feel free to reach out.
Q: How to process the mass data?
A: For data analysis, we provide Thermo Compound Discoverer software. Recorded training sessions from the manufacturer are available for viewing, but due to copyright reasons, direct access is only possible at the core laboratory.
Alternatively, the MS data can also be processed using publicly available software, such as XCMS, mzMine, MS-DIAL, or Skyline.